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KMID : 1025520090510010001
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Journal of Animal Science and Technology
2009 Volume.51 No. 1 p.1 ~ p.8
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Functional Analysis of expressed Sequence Tags from Hanwoo (Korean Cattle) cDNA Libraries
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Lim Da-Jeong
Yoon Du-Hak
Cho Yong-Min
Byun Mi-Jeong
Lee Seung-Hwan
Shin Youn-Hee
Im Seok-Ki
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Abstract
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We generated 57,598 expressed sequence tags(ESTs) from 3 cDNA libraries of Hanwooo(Korean Cattle), fat, loin, liver. Liver, intermuscular fat and longissimus dorsi tissues were obtained from a 24-month-old Hanwoo steer immediately after slaughter. cDNA library was constructed according to the oligocapped method. The EST data were clustered and assembled into unique sequences, 4,759 contigs and 7,587 singletons. To carry out functional analysis, Gene Ontology annotation and identification of significant leaf nodes were performed that were detected by searching significant p-values from 2nd level GO terms to leaf nodes using Bonferroni correction. We found that 13, 26 and 8 significant leaf nodes are unique in the transcripts according to 3 GO categories, molecular function, biological process and cellular component. Also digital gene expression profiling using the Audic’s test was performed and tissue specific genes were detected in the above 3 libraries.
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KEYWORD
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Hanwoo, EST, Gene Ontology, Audic`s test
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